BehaviorAtlas Optomotor · Software Suite

Mouse Optomotor Analysis System · Software Suite

Precise OMR head movement capture with flexible visual stimulation — quantifying spatial frequency and contrast sensitivity thresholds for vision and neuroscience research.

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Software Functions

BehaviorAtlas Optomotor Capture Mouse — stimulus control, OMR/OKR behavior recording, and multimodal synchronization.

Flexible Stimulus Parameters

Customize spatial frequency, contrast sensitivity (0–1), grating texture (sine/square/sawtooth), and stimulus direction for dynamic optomotor scenes.

OMR Head Movement Capture

Top camera records head and body movement during optomotor stimulation — high-quality raw video for OMR analysis.

AI-powered OKR Eye Tracking

AI tracks pupil trajectories at millisecond resolution for optokinetic response (OKR) during grating motion — significantly improving detection precision.

Multimodal Synchronized Capture

Integrates with BA-EyeTracking for synchronized eye-tracking and head movement data — multi-dimensional support for visual function assessment.

TTL Event Synchronization

Event sync module records timestamps and sync I/O signals — aligning optomotor stimuli, video, and external peripherals.

Spatial Frequency Threshold Assays

Stepwise spatial frequency adjustment to quantify visual acuity (VA) threshold — measuring fine pattern resolution capacity.

Contrast Sensitivity Threshold Assays

Adjust contrast at fixed spatial frequency to measure contrast sensitivity (CS) threshold — resolution in low-contrast conditions.

Stimulus Preview

Preview grating motion after parameter setup — verify frequency, contrast, and texture before recording.

Non-invasive Natural-state Recording

Unrestrained mice on elevated platform — non-invasive recording that reflects natural visual function without surgery or fixation.

Broad Research Applications

For ophthalmic disease models, drug efficacy assessment, visual development mechanisms, and neurodegenerative vision research.

Software Matrix

Optomotor Capture and Optomotor Analyzer — acquisition and data analysis for optomotor experiments.

BehaviorAtlas Optomotor Capture Mouse
Capture

OMR

Head tracking

TTL

Event sync

Stimulus parameter setup, preview, video recording, and TTL marking — flexible spatial frequency, contrast, grating texture, and direction with synchronized OMR head movement video.

BehaviorAtlas Optomotor Capture Mouse

Visual stimulus control & behavior acquisition

In Development

Analysis module under active development

Analyze

VA/CS

Threshold

Coming soon

Analyzer

Automated optomotor experiment analysis — OMR head movement analysis, body keypoint tracking, spatial frequency/contrast sensitivity thresholds, visualization, and report export. (In development)

BehaviorAtlas Optomotor Analyzer Mouse

Automated optomotor data analysis

Workflow

Dark adaptation and placement, parameter config, acquisition and stimulation — AI tracks OMR head movement to output visual thresholds.

Experiment Preparation

After dark adaptation, place mouse on central platform in optomotor apparatus for free movement before experiment.

  • Dark adaptation duration per experimental protocol
  • Place animal after health check approval
  • Head freely mobile, no fixation required

Create a grating stimulus event in capture software — configure spatial frequency, contrast, opacity, grating texture, direction, and moving speed, then lock the gradient protocol after preview.

  • Adjustable spatial frequency (cpd), contrast (0–1), and grating texture
  • Stimulus direction, moving speed, duration, and refresh interval
  • Lock protocol after full-screen preview confirmation

Experiment Execution

Top behavior camera records head and body movement synchronized with grating stimulus timeline.

  • Full-field coverage of experiment area
  • Continuous head and body movement video recording
  • Synchronized marking with stimulus parameters

Visual stimulation begins in preset sequence on six-sided surround displays — gratings presented in configured temporal order, direction, and duration.

  • Six-sided immersive optomotor environment
  • Alternating stimulus direction cycles
  • TTL peripheral synchronized triggering

AI algorithms analyze head pose to determine whether head movement tracks the rotating grating.

  • Head: tracking determined by ear-center and nose-tip keypoints
  • Automatic distinction of non-specific head movement interference
  • Head movement data aligned with stimulus timeline

Data Analysis

Aggregate head movement tracking trajectories — AI auto-determines tracking status, outputs spatial frequency and contrast sensitivity threshold reports.

  • Synchronized head movement trajectory recording
  • Automatic tracking/non-tracking state determination
  • Export OMR results, body keypoint tracking data, and statistical charts

Analysis Results

From standardized trajectories and OMR detection to visualization export — traceable optomotor analysis outputs.

OMR Data Analysis Results

01

OMR Data Analysis Results

Trajectories OMR detection H5 export

Three output layers — standardized keypoint and smoothed trajectories with per-trial valid frames; OMR segment detection using head-direction consistency, sustained frames, head-length stability, and grooming filtering; plus annotated review videos and H5 files for QC, statistics, and verification.

Optomotor Result Review & QC

02

Optomotor Result Review & QC

Frame review Keypoint tracking Data QC

Per-sample review syncs stimulus timelines with whole-body and keypoint tracking videos (nose, ears, neck, back), plus trajectory and speed curves — frame-by-frame OMR verification for fast data QC and result confirmation.

Data Visualization

03

Data Visualization

Single sample Group analysis Behavior spectrum

Single-sample OMR visualization plus multi-sample group statistics — inter-group time proportions, head-following behavior spectra, VA/CS threshold charts, and more for group comparison and publication-ready export.

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Book an online or on-site demo — experience the full workflow with tailored protocol advice.

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